Using T cell lymphokines of hyperimmunized chickens with Salmonella pullorum to protect layer hens against Salmonella pullorum infection

نویسندگان

چکیده

Salmonella pullorum (SP) is well adapted to cause an acute systemic infection in hens with a high mortality rate. This study aimed assess the efficacy and safety of salmonella-immune lymphokine (SILK) produced by hyperimmunized pullets during layer hens' production stage boost their immune response against infection. In this study, two groups 25 were used produce lymphokines; first group received three doses vaccine at 12, 14, 16 weeks, while second only phosphate buffer saline (PBS), which served as control. At 18 non-immune lymphokines (NILK) isolated from T cells group, group. Then, 100-layer (ISSA Brown), 30 weeks old, entered stage, separated into four groups, each chickens. G1: infected SP treated SILK. G2: NILK. G3: untreated SP-challenged G4: no treatment or challenge. The current describe host humoral responses serum samples bacterial shedding challenged 31-33 qPCR techniques. Internal organ loads estimated evaluate persistence bacteria. results show that spleen, liver, caecal tonsils tested positive for bacteria both (G2, G3), proving was not eliminated birds suggesting internal colonization may act reservoir ongoing low IgG titer compared G1 extremely 31, 32, 33. investigation shows using SILK provides better homogenous protection less persistence.

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ژورنال

عنوان ژورنال: Iraqi journal of Veterinary Sciences

سال: 2022

ISSN: ['2071-1255', '1607-3894']

DOI: https://doi.org/10.33899/ijvs.2022.136020.2555